ABSTRACT
Objective To explore the relationship between the expression of VEGF and TGF-β1 in naso-pharyngeal carcinoma and the clinical characteristics and prognosis of nasopharyngeal carcinoma patients. Meth-ods The expression of VEGF and TGF-β1 in the local middle-late nasopharyngeal carcinoma tissue of 96 patients was detected using immunohistochemistry.The correlation and the prognosis analysis were analyzed in combination with their clinical characteristics and prognosis. Results Expression rate of VEGF was 66.7% and that of TGF-β 1 53.1%.The expression of VEGF and TGF-β1 were positively correlated in the advanced nasopharyngeal carcino-ma.Both VEGF and TGF-β1 had significant correlation with lymphatic metastasis.The effects of VEGF and TGF-β1 on MFS were analyzed by Cox single factor and the results showed that VEGF positive was an adverse prognostic factor(P<0.05).The effects of age,sex,lymphatic metastasis,local invasion,VEGF and TGF-β1 on MFS were analyzed with Cox multifactor analysis and the results showed that VEGF positive was an adverse prognostic factor (P < 0.01). Conclusion The detection of VEGF and TGF-β1 protein in nasopharyngeal carcinoma may help to predict the metastasis of nasopharyngeal carcinoma.
ABSTRACT
Objective To study the relationship between Treg cell numbers and level of TGF-β1,IL-10 in the immune microenvironment of rat liver cancer.Method 40 male Wistar rats were randomly divided into three groups,liver cancer model group (n =20),saline control group (n =10) and blank control group (n =10).Liver cancer model was established by intraperitoneal injection of DEN (100 mg/kg).Percentage of Treg cells in cancer tissues was detected by fiow cytometry and the expression level of TGF-β1 and IL-10 by immunohistochemical SABC.Results The ratio of Treg cells in CD4 + T cells was 14.32％ ± 4.84％ in liver cancer tissues,significantly higher than that in the blank control group 5.64％ ±6.10％ and the saline control group 7.95％ ±3.55％.The difference was statistically significant (F =211.279,P ＜ 0.05).The expression level of TGF-β1 and IL-10 in cancer tissues was significantly higher than the blank control group and the saline control group (FTGF-β1 =250.740,FIL-10 =152.744,all P ＜0.05).The number of Treg cells was positively correlated with TGF-β1,IL-10 level (P ＜ 0.05).Conclusions TGF-β1,IL-10 and Treg cells significantly increased in rat experimental liver cancer tissues,and there was positive correlation between Treg cells,and TGF-β1 and IL-10 in liver cancer tissues.